RNase A

Thương hiệu: Bio Basic-Canada   |   Tình trạng: Còn hàng
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RNase A
CAT: RB0473
CAS: 9001-99-4
Grade: Biotech
DG: No
Storage: (-15 to -20)C

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RNase A
CAT: RB0473
CAS: 9001-99-4
Grade: Biotech
DG: No
Storage: (-15 to -20)C
Sterile: Yes
Stock: Usually in Stock

Product Description: RNase A: Product Description A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA. In this application, the presence of DNase activity as an impurity is a concern. The boiling-water bath method used to eliminate contaminating DNase activity has proven unreliable. For this reason, Bio Basic Inc. developed a proprietary chromatographic preparation method for elimination of DNase activity.
RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119 , which are present in the active site of the enzyme. Activators of RNase.

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